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|Title:||Metabolite profiling of Clostridium difficile ribotypes using small molecular weight volatile organic compounds|
Clokie, M. R. J.
Ellis, A. M.
Monks, P. S.
|Publisher:||Springer Verlag for Metabolomics Society|
|Citation:||Metabolomics, 2015, 11 (2), pp. 251-260|
|Abstract:||Volatile organic compounds (VOCs) emitted by cultures of ten different Clostridium difficile ribotypes have been profiled using proton transfer reaction-time of flight-mass spectrometry. A total of 69 VOCs were identified and combinations of these VOCs were found to be characteristic for each of the ribotypes. The VOC patterns, with the aid of a statistical analysis, have been shown to be useful in distinguishing different ribotypes. A tentative assignment of different masses also shows that different ribotypes have markedly different emissions of methanol, p-cresol, dimethylamine and a range sulfur compounds (ethylene sulfide, dimethylsulfide and methyl thioacetate), which point to VOCs as potential indicators of different metabolic pathways in virulent and less-virulent strains. The results establish the potential of detecting emitted VOC metabolites to differentiate between closely related C. difficile ribotypes and in the longer term provide metabolic insight into virulence.|
|Rights:||Copyright © 2014, Springer Verlag for Metabolomics Society. Deposited with reference to the publisher’s open access archiving policy.|
|Appears in Collections:||Published Articles, Dept. of Chemistry|
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|Cdiff paper ref rev v1 no track.pdf||Post-review (final submitted author manuscript)||2.31 MB||Adobe PDF||View/Open|
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