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Title: Metabolite profiling of Clostridium difficile ribotypes using small molecular weight volatile organic compounds
Authors: Kuppusami, S.
Clokie, M. R. J.
Panayi, T.
Ellis, A. M.
Monks, P. S.
First Published: 17-Jul-2014
Publisher: Springer Verlag for Metabolomics Society
Citation: Metabolomics, 2015, 11 (2), pp. 251-260
Abstract: Volatile organic compounds (VOCs) emitted by cultures of ten different Clostridium difficile ribotypes have been profiled using proton transfer reaction-time of flight-mass spectrometry. A total of 69 VOCs were identified and combinations of these VOCs were found to be characteristic for each of the ribotypes. The VOC patterns, with the aid of a statistical analysis, have been shown to be useful in distinguishing different ribotypes. A tentative assignment of different masses also shows that different ribotypes have markedly different emissions of methanol, p-cresol, dimethylamine and a range sulfur compounds (ethylene sulfide, dimethylsulfide and methyl thioacetate), which point to VOCs as potential indicators of different metabolic pathways in virulent and less-virulent strains. The results establish the potential of detecting emitted VOC metabolites to differentiate between closely related C. difficile ribotypes and in the longer term provide metabolic insight into virulence.
DOI Link: 10.1007/s11306-014-0692-4
ISSN: 1573-3882
eISSN: 1573-3890
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014, Springer Verlag for Metabolomics Society. Deposited with reference to the publisher’s open access archiving policy.
Appears in Collections:Published Articles, Dept. of Chemistry

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