Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/40153
Title: Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control
Authors: Danilenko, Marina
Dalgliesh, Caroline
Pagliarini, Vittoria
Naro, Chiara
Ehrmann, Ingrid
Feracci, Mikael
Kheirollahi-Chadegani, Mahsa
Tyson-Capper, Alison
Clowry, Gavin J.
Fort, Philippe
Dominguez, Cyril
Sette, Claudio
Elliott, David J.
First Published: 20-Dec-2016
Publisher: Oxford University Press (OUP)
Citation: Nucleic Acids Research, 2017, 45 (7), pp. 4120-4130
Abstract: SLM2 and Sam68 are splicing regulator paralogs that usually overlap in function, yet only SLM2 and not Sam68 controls the Neurexin2 AS4 exon important for brain function. Herein we find that SLM2 and Sam68 similarly bind to Neurexin2 pre-mRNA, both within the mouse cortex and in vitro. Protein domain-swap experiments identify a region including the STAR domain that differentiates SLM2 and Sam68 activity in splicing target selection, and confirm that this is not established via the variant amino acids involved in RNA contact. However, far fewer SLM2 and Sam68 RNA binding sites flank the Neurexin2 AS4 exon, compared with those flanking the Neurexin1 and Neurexin3 AS4 exons under joint control by both Sam68 and SLM2. Doubling binding site numbers switched paralog sensitivity, by placing the Neurexin2 AS4 exon under joint splicing control by both Sam68 and SLM2. Our data support a model where the density of shared RNA binding sites around a target exon, rather than different paralog-specific protein-RNA binding sites, controls functional target specificity between SLM2 and Sam68 on the Neurexin2 AS4 exon. Similar models might explain differential control by other splicing regulators within families of paralogs with indistinguishable RNA binding sites.
DOI Link: 10.1093/nar/gkw1277
ISSN: 0305-1048
eISSN: 1362-4962
Links: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw1277
http://hdl.handle.net/2381/40153
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2016. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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