Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/40172
Title: Randomized trial of red cell washing for the prevention of transfusion-associated organ injury in cardiac surgery
Authors: Woźniak, Marcin J.
Sullo, N.
Qureshi, S.
Dott, W.
Cardigan, R.
Wiltshire, M.
Morris, T.
Nath, M.
Bittar, N.
Bhudia, S. K.
Kumar, T.
Goodall, A. H.
Murphy, G. J.
First Published: 15-May-2017
Publisher: Oxford University Press (OUP)
Citation: British Journal of Anaesthesia, 2017, 118 (5), pp. 689-698
Abstract: Background: Experimental studies suggest that mechanical cell washing to remove pro-inflammatory components that accumulate in the supernatant of stored donor red blood cells (RBCs) might reduce inflammation and organ injury in transfused patients. Methods: Cardiac surgery patients at increased risk of large-volume RBC transfusion were eligible. Participants were randomized to receive either mechanically washed allogenic RBCs or standard care RBCs. The primary outcome was serum interleukin-8 measured at baseline and at four postsurgery time points. A mechanism substudy evaluated the effects of washing on stored RBCs in vitro and on markers of platelet, leucocyte, and endothelial activation in trial subjects. Results: Sixty adult cardiac surgery patients at three UK cardiac centres were enrolled between September 2013 and March 2015. Subjects received a median of 3.5 (interquartile range 2-5.5) RBC units, stored for a mean of 21 ( sd 5.2) days, within 48 h of surgery. Mechanical washing reduced concentrations of RBC-derived microvesicles but increased cell-free haemoglobin concentrations in RBC supernatant relative to standard care RBC supernatant. There was no difference between groups with respect to perioperative serum interleukin-8 values [adjusted mean difference 0.239 (95% confidence intervals -0.231, 0.709), P =0.318] or concentrations of plasma RBC microvesicles, platelet and leucocyte activation, plasma cell-free haemoglobin, endothelial activation, or biomarkers of heart, lung, or kidney injury. Conclusions: These results do not support a hypothesis that allogenic red blood cell washing has clinical benefits in cardiac surgery. Clinical trial registration: ISRCTN 27076315.
DOI Link: 10.1093/bja/aex083
ISSN: 0007-0912
eISSN: 1471-6771
Links: https://academic.oup.com/bja/article-lookup/doi/10.1093/bja/aex083
http://hdl.handle.net/2381/40172
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Cardiovascular Sciences

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