Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/40268
Title: MUC5AC and a Glycosylated Variant of MUC5B Alter Mucin Composition in Children With Acute Asthma.
Authors: Welsh, Kathryn G.
Rousseau, Karine
Fisher, Gemma
Bonser, Luke R.
Bradding, Peter
Brightling, Chris E.
Thornton, David J.
Gaillard, Erol A.
First Published: 14-Jul-2017
Publisher: Elsevier, American College of Chest Physicians (ACCP)
Citation: Chest, 2017
Abstract: BACKGROUND: Diffuse airway mucus obstruction is an important feature of severe and fatal asthma. MUC5AC and MUC5B are the principal gel-forming mucins found in airway mucus. The mucin composition of airway mucus likely affects its functional properties. METHODS: We quantified the principal airway mucins MUC5AC and MUC5B in the sputum of age-matched children with acute and stable asthma and healthy control subjects by using Western blotting. RESULTS: Sputum samples from 38 children (13 with acute asthma, 15 with stable asthma, 10 control subjects) were obtained. Sputum MUC5AC concentrations were 7.6 μg/mL in control subjects, 22.4 μg/mL in those with stable asthma (P = .17), and 44.7 μg/mL in those with acute asthma (P < .05). MUC5B concentrations showed less variation, with 156.2, 222.3, and 254.8 μg/mL in control subjects, those with stable asthma, and those with acute asthma, respectively. The greater MUC5AC concentration in those with acute asthma resulted in a significantly altered MUC5B:MUC5AC ratio between control subjects and those with acute asthma (P < .05). Significant differences in MUC5B glycoforms were present between the groups, with the low-charge-only glycoform being found uniquely in those with acute asthma. CONCLUSIONS: Increased MUC5AC and the presence of a low-charge-only MUC5B glycoform significantly altered mucin composition in children with acute asthma. These changes may be important contributory factors to the airway mucus obstruction observed during acute asthma.
DOI Link: 10.1016/j.chest.2017.07.001
ISSN: 0012-3692
eISSN: 1931-3543
Links: http://www.sciencedirect.com/science/article/pii/S0012369217312424
http://hdl.handle.net/2381/40268
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: The e-Appendix and e-Figures can be found in the Supplemental Materials section of the online article.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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