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Title: Development of competitive 'pseudo'-ELISA assay for measurement of cocaine and its metabolites using molecularly imprinted polymer nanoparticles
Authors: Garcia, Yadiris
Smolinska-Kempisty, Katarzhina
Pereira, Eduardo
Piletska, Elena
Piletsky, Sergey
First Published: 14-Jul-2017
Publisher: Royal Society of Chemistry
Citation: Analytical Methods, 2017, 9 (31), pp. 4592-4598
Abstract: The analytical test-system for cocaine, benzoylecgonine and norcocaine was developed in the ELISA format using molecularly imprinted polymeric nanoparticles (nanoMIPs) as synthetic recognition elements that were produced using a solid-phase synthesis approach. The experimental conditions of the assay were optimized using a Box-Behnken experimental design protocol. The detection of free cocaine and its metabolites was performed using a competitive binding assay in the model samples and in blood plasma. There was no cross-reactivity of the developed assay towards paracetamol and caffeine. The developed assay had a picomolar limit of detection of cocaine (LOD = 4.24 pM), which was almost three orders of magnitude lower than the LOD expected from the commercial antibody-based ELISA (3.3 nM), and other attractive features of a new assay included a long shelf-life, lower economic cost and a short production time. Therefore, it is possible to state that nanoMIPs have the potential to become the recognition elements of choice for the development of a new generation of test-systems and sensors.
DOI Link: 10.1039/c7ay01523b
ISSN: 1759-9660
eISSN: 1759-9679
Embargo on file until: 14-Jul-2018
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2017, Royal Society of Chemistry. Deposited with reference to the publisher’s open access archiving policy.
Description: The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.
Appears in Collections:Published Articles, Dept. of Chemistry

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