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|Title:||Structural Basis of the Mispairing of an Artificially Expanded Genetic Information System|
|Authors:||Reichenbach, Linus F.|
Sobri, Ahmad Ahmad
Zaccai, Nathan R.
Eperon, Lucy P.
de Ornellas, Sara
Eperon, Ian C.
Brady, R. Leo.
Burley, Glenn A.
|Publisher:||Elsevier (Cell Press)|
|Citation:||Chem, 2016, 1 (6), pp. 946-958|
|Abstract:||Relative to naturally occurring Watson-Crick base pairs, the synthetic nucleotide P pairs with Z within DNA duplexes through a unique hydrogen-bond arrangement. The loss of this synthetic genetic information by PCR results in the conversion of P-Z into a G-C base pair. Here, we show structural and spectroscopic evidence that the loss of this synthetic genetic information occurs via G-Z mispairing. Remarkably, the G-Z mispair is both plastic and pH dependent; it forms a double-hydrogen-bonded “slipped” pair at pH 7.8 and a triple-hydrogen-bonded Z-G pair when the pH is above 7.8. This study highlights the need for robust structural and functional methods to elucidate the mechanisms of mutation in the development of next-generation synthetic genetic base pairs.|
|Rights:||Copyright © 2016, Elsevier (Cell Press). Deposited with reference to the publisher’s open access archiving policy.|
|Description:||The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.|
|Appears in Collections:||Published Articles, Dept. of Molecular and Cell Biology|
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|Reichenbach_Chem_2016.pdf||Post-review (final submitted author manuscript)||5.94 MB||Adobe PDF||View/Open|
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