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Title: Structural Basis of the Mispairing of an Artificially Expanded Genetic Information System
Authors: Reichenbach, Linus F.
Sobri, Ahmad Ahmad
Zaccai, Nathan R.
Agnew, Christopher
Burton, Nicholas
Eperon, Lucy P.
de Ornellas, Sara
Eperon, Ian C.
Brady, R. Leo.
Burley, Glenn A.
First Published: 8-Dec-2016
Publisher: Elsevier (Cell Press)
Citation: Chem, 2016, 1 (6), pp. 946-958
Abstract: Relative to naturally occurring Watson-Crick base pairs, the synthetic nucleotide P pairs with Z within DNA duplexes through a unique hydrogen-bond arrangement. The loss of this synthetic genetic information by PCR results in the conversion of P-Z into a G-C base pair. Here, we show structural and spectroscopic evidence that the loss of this synthetic genetic information occurs via G-Z mispairing. Remarkably, the G-Z mispair is both plastic and pH dependent; it forms a double-hydrogen-bonded “slipped” pair at pH 7.8 and a triple-hydrogen-bonded Z-G pair when the pH is above 7.8. This study highlights the need for robust structural and functional methods to elucidate the mechanisms of mutation in the development of next-generation synthetic genetic base pairs.
DOI Link: 10.1016/j.chempr.2016.11.009
ISSN: 2451-9294
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2016, Elsevier (Cell Press). Deposited with reference to the publisher’s open access archiving policy.
Description: The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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