Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/40390
Full metadata record
DC FieldValueLanguage
dc.contributor.authorReichenbach, Linus F.-
dc.contributor.authorSobri, Ahmad Ahmad-
dc.contributor.authorZaccai, Nathan R.-
dc.contributor.authorAgnew, Christopher-
dc.contributor.authorBurton, Nicholas-
dc.contributor.authorEperon, Lucy P.-
dc.contributor.authorde Ornellas, Sara-
dc.contributor.authorEperon, Ian C.-
dc.contributor.authorBrady, R. Leo.-
dc.contributor.authorBurley, Glenn A.-
dc.date.accessioned2017-09-21T16:08:49Z-
dc.date.available2017-12-08T02:45:06Z-
dc.date.issued2016-12-08-
dc.identifier.citationChem, 2016, 1 (6), pp. 946-958en
dc.identifier.issn2451-9294-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S2451929416302364?via%3Dihuben
dc.identifier.urihttp://hdl.handle.net/2381/40390-
dc.descriptionThe file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.en
dc.description.abstractRelative to naturally occurring Watson-Crick base pairs, the synthetic nucleotide P pairs with Z within DNA duplexes through a unique hydrogen-bond arrangement. The loss of this synthetic genetic information by PCR results in the conversion of P-Z into a G-C base pair. Here, we show structural and spectroscopic evidence that the loss of this synthetic genetic information occurs via G-Z mispairing. Remarkably, the G-Z mispair is both plastic and pH dependent; it forms a double-hydrogen-bonded “slipped” pair at pH 7.8 and a triple-hydrogen-bonded Z-G pair when the pH is above 7.8. This study highlights the need for robust structural and functional methods to elucidate the mechanisms of mutation in the development of next-generation synthetic genetic base pairs.en
dc.language.isoenen
dc.publisherElsevier (Cell Press)en
dc.rightsCopyright © 2016, Elsevier (Cell Press). Deposited with reference to the publisher’s open access archiving policy.en
dc.titleStructural Basis of the Mispairing of an Artificially Expanded Genetic Information Systemen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.chempr.2016.11.009-
dc.description.statusPeer-revieweden
dc.description.versionPost-printen
dc.type.subtypeJournal Article-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departmentsen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Molecular & Cell Biologyen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Canceren
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Genome Scienceen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Molecular & Cellular Bioscienceen
dc.dateaccepted2016-11-16-
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

Files in This Item:
File Description SizeFormat 
Reichenbach_Chem_2016.pdfPost-review (final submitted author manuscript)5.94 MBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.