Please use this identifier to cite or link to this item:
|Title:||Orchestrated Domain Movement in Catalysis by Cytochrome P450 Reductase|
|Authors:||Freeman, Samuel L.|
Raven, Emma L.
Roberts, Gordon C. K.
|Publisher:||Nature Publishing Group|
|Citation:||Scientific Reports, 2017, 7 (1), Article number: 9741|
|Abstract:||NADPH-cytochrome P450 reductase is a multi-domain redox enzyme which is a key component of the P450 mono-oxygenase drug-metabolizing system. We report studies of the conformational equilibrium of this enzyme using small-angle neutron scattering, under conditions where we are able to control the redox state of the enzyme precisely. Different redox states have a profound effect on domain orientation in the enzyme and we analyse the data in terms of a two-state equilibrium between compact and extended conformations. The effects of ionic strength show that the presence of a greater proportion of the extended form leads to an enhanced ability to transfer electrons to cytochrome c. Domain motion is intrinsically linked to the functionality of the enzyme, and we can define the position of the conformational equilibrium for individual steps in the catalytic cycle.|
|Rights:||Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Appears in Collections:||Published Articles, Dept. of Chemistry|
Files in This Item:
|41598_2017_Article_9840.pdf||Published (publisher PDF)||3.13 MB||Adobe PDF||View/Open|
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.