Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/40537
Title: Unique apicomplexan IMC sub-compartment proteins are early markers for apical polarity in the malaria parasite
Authors: Poulin, Benoit
Patzewitz, Eva-Maria
Brady, Declan
Silvie, Olivier
Wright, Megan H.
Ferguson, David J.P.
Wall, Richard J.
Whipple, Sarah
Guttery, David S.
Tate, Edward W.
Wickstead, Bill
Holder, Anthony A.
Tewari, Rita
First Published: 15-Nov-2013
Publisher: Company of Biologists
Citation: Biology Open, 2013, 2 (11), pp. 1160-1170
Abstract: The phylum Apicomplexa comprises over 5000 intracellular protozoan parasites, including Plasmodium and Toxoplasma, that are clinically important pathogens affecting humans and livestock. Malaria parasites belonging to the genus Plasmodium possess a pellicle comprised of a plasmalemma and inner membrane complex (IMC), which is implicated in parasite motility and invasion. Using live cell imaging and reverse genetics in the rodent malaria model P. berghei, we localise two unique IMC sub-compartment proteins (ISPs) and examine their role in defining apical polarity during zygote (ookinete) development. We show that these proteins localise to the anterior apical end of the parasite where IMC organisation is initiated, and are expressed at all developmental stages, especially those that are invasive. Both ISP proteins are N-myristoylated, phosphorylated and membrane-bound. Gene disruption studies suggest that ISP1 is likely essential for parasite development, whereas ISP3 is not. However, an absence of ISP3 alters the apical localisation of ISP1 in all invasive stages including ookinetes and sporozoites, suggesting a coordinated function for these proteins in the organisation of apical polarity in the parasite.
DOI Link: 10.1242/bio.20136163
ISSN: 2046-6390
Links: http://bio.biologists.org/content/2/11/1160
http://hdl.handle.net/2381/40537
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2013. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

Files in This Item:
File Description SizeFormat 
Unique apicomplexan IMC sub-compartment proteins are early markers for apical polarity in the malaria parasite.pdfPublished (publisher PDF)1.08 MBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.