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|Title:||B cell O-galactosyltransferase activity, and expression of O-glycosylation genes in bone marrow in IgA nephropathy|
|Authors:||Buck, Katharine S.|
Smith, Alice C.
|Publisher:||Nature Publishing Group|
|Citation:||Kidney International, 2008, 73 (10), pp. 1128-1136|
|Abstract:||In IgA nephropathy pathogenic IgA1 is likely derived from bone marrow cells and exhibits reduced O-galactosylation. Defective O-galactosylation may arise from compromised expression or function of the enzyme β-galactosyltransferase and/or its molecular chaperone (Cosmc). We measured B cell O-galactosylation activity and the relative gene expression of β-galactosyltransferase and Cosmc in peripheral blood and bone marrow taken from patientswith IgA nephropathy and controls. O-galactosylation activity was measured in peripheral and bone marrow cells controls by the incorporation of radiolabelled galactose into an asialo-mucin acceptor. Gene expression of β-galactosyltransferase and Cosmc was measured by real time PCR and related to that of the enzyme GalNAc-T2, which synthesizes the core O-glycan. Neither the B cell O-galactosylation activity nor the gene expression of the enzyme or chaperone were different between patients and controls. However, the relationships between the O-glycosylation of serum IgA1, galactosylation activity and β-galactosyltransferase gene expression showed different patterns in IgA nephropathy and controls. In IgA nephropathy, O-galactosylation activity correlated with β-galactosyltransferase gene expression, but not with IgA1 O-glycosylation, suggesting that factors other than the availability of β-galactosyltransferase or Cosmc are responsible for altered IgA1 O-glycosylation.|
|Rights:||This is the author's final draft of the paper published as Kidney International, 2008, 73 (10), pp. 1128-1136. The final version is available from http://www.nature.com/ki/journal/v73/n10/abs/5002748a.html. Doi: 10.1038/sj.ki.5002748|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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