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Title: Short communication: Towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the elaeis genus
Authors: Zaki, Noorharzia Mohd
Singh, Rajinder
Nor, Nordiana Hanim Mohd
Zulkifli, Muhammad Azwan
Smith, Steven W.
Schwarzacher, Trude
Madon, M.
Heslop-Harrison, J. S.
First Published: 29-Dec-2017
Publisher: Malaysian Palm Oil Board
Citation: Journal of Oil Palm Research, 2017, 29 (4), pp. 594-599
Abstract: The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore, the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.
DOI Link: 10.21894/jopr.2017.0001
ISSN: 1511-2780
Embargo on file until: 1-Jan-10000
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2017, Malaysian Palm Oil Board. Deposited with reference to the publisher’s open access archiving policy. (
Description: The file associated with this record is under a permanent embargo in accordance with the publisher's policy. The full text may be available through the publisher links provided above.
Appears in Collections:Published Articles, Dept. of Biology

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