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|Title:||Short communication: Towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the elaeis genus|
|Authors:||Zaki, Noorharzia Mohd|
Nor, Nordiana Hanim Mohd
Zulkifli, Muhammad Azwan
Smith, Steven W.
Heslop-Harrison, J. S.
|Publisher:||Malaysian Palm Oil Board|
|Citation:||Journal of Oil Palm Research, 2017, 29 (4), pp. 594-599|
|Abstract:||The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore, the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.|
|Embargo on file until:||1-Jan-10000|
|Rights:||Copyright © 2017, Malaysian Palm Oil Board. Deposited with reference to the publisher’s open access archiving policy. (http://www.rioxx.net/licenses/all-rights-reserved)|
|Description:||The file associated with this record is under a permanent embargo in accordance with the publisher's policy. The full text may be available through the publisher links provided above.|
|Appears in Collections:||Published Articles, Dept. of Biology|
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|ElaeisChrMarkers2017.pdf||Post-review (final submitted author manuscript)||1.18 MB||Adobe PDF||View/Open|
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