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|Title: ||Biological Ageing Effect of Radiotherapy in Breast Cancer|
|Authors: ||Mohammed, A.|
Almeida, Gabriela M.
Talbot, Christopher J.
Royle, Nicola J.
Jones, George D.D.
Barwell, Julian G.
|Issue Date: ||2008|
|Publisher: ||BMJ Publishing Group|
|Citation: ||Poster Presentation 5.10 at the British Human Genetics Conference 2008, Abstract Booklet published as a supplement to the Journal of Medical Genetics, pp. S116.|
|Abstract: ||The ability of peripheral blood lymphocytes to undergo radiation-induced apoptosis in vitro
usually falls by only 0.5% per year. In a recent study, it has been found that in vitro apoptotic
response to ionising radiation in peripheral blood lymphocytes fell by 15% in 12 breast cancer
patients when repeated one year post radiotherapy (Docherty et al, 2007). This is equivalent
to 30 years of biological ageing over a one year period post irradiation. Our hypothesis is that
the decrease in peripheral blood lymphocytes ability to undergo apoptosis is a result of
irradiation-induced biological ageing. This project aims to validate proposed in vitro markers
of biological ageing in peripheral blood lymphocytes (including global genomic Methylation by
High Performance Liquid Chromatography analysis and Telomere Lengths using Single
Telomere Length Analysis and Telomere Restriction Fragment analysis) and radiosensitivity
assays (including radiation-induced apoptotic response via Sub-G1 and Annexin V/FITC
assays, and DNA Single Strand Break & Double Strand Break formation and repair via Comet
and gamma-H2AX assays). We plan to investigate these observations within a larger breast
cancer patient and control cohort to investigate the cause of biological ageing. It is hoped that
these assays could be used to predict response to treatment/complications or prognosis, or to
determine the effectiveness of bio-preventative agents in the future.|
|Description: ||This is the author's final draft of Poster Presentation 5.10 at the British Human Genetics Conference, 2008. The Abstract Booklet is published as a supplement to the Journal of Medical Genetics. The final version is available from http://jmg.bmj.com/|
|Appears in Collections:||Conference Papers & Presentations, Dept. of Genetics|
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