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Title: Use of Vectorette and Subvectorette PCR to Isolate Transgene Flanking DNA
Authors: Allen, Maxine J.
Collick, Andrew
Jeffreys, Alec J.
First Published: Oct-1994
Publisher: Cold Spring Harbor Laboratory Press.
Citation: Genome Research, 1994, 4 (2), pp. 71-75.
Abstract: Vectorette PCR permits the specific amplification of DNA segments flanking a known DNA sequence. It enables the application of the PCR where sequence information is only available for one primer site. We now show that vectorette PCR can be used for the systematic mapping and retrieval of transgene flanking DNA. We also show that the sequence of large vectorette PCR fragments can be obtained without cloning, by the production of subvectorette fragments.
DOI Link: 10.1101/gr.4.2.71
ISSN: 1054-9803
Type: Article
Rights: Copyright © 1994, Cold Spring Harbor Laboratory Press Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License,
Appears in Collections:Published Articles, Dept. of Genetics

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