Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/43089
Title: Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Authors: Roversi, Pietro
Johnson, Steven
Preston, Stephen G.
Nunn, Miles A.
Paesen, Guido C.
Austyn, Jonathan M.
Nuttall, Patricia A.
Lea, Susan M.
First Published: 22-Nov-2017
Publisher: Nature Research (part of Springer Nature)
Citation: Scientific Reports, 2017, 7:16057
Abstract: Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives.
DOI Link: 10.1038/s41598-017-16413-2
eISSN: 2045-2322
Links: https://www.nature.com/articles/s41598-017-16413-2
http://hdl.handle.net/2381/43089
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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