Please use this identifier to cite or link to this item:
Title: Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Authors: Roversi, Pietro
Johnson, Steven
Preston, Stephen G.
Nunn, Miles A.
Paesen, Guido C.
Austyn, Jonathan M.
Nuttall, Patricia A.
Lea, Susan M.
First Published: 22-Nov-2017
Publisher: Nature Research (part of Springer Nature)
Citation: Scientific Reports, 2017, 7:16057
Abstract: Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives.
DOI Link: 10.1038/s41598-017-16413-2
eISSN: 2045-2322
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

Files in This Item:
File Description SizeFormat 
Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.pdfPublished (publisher PDF)1.73 MBAdobe PDFView/Open

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.