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Title: Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants.
Authors: Higo, Asuka
Kawashima, Tomokazu
Borg, Michael
Zhao, MMingmin
López-Vidriero, Irene
Sakayama, Hidetoshi
Montgomery, Sean A.
Sekimoto, Hiroyuki
Hackenberg, Dieter
Shimamura, Masaki
Nishiyama, Tomoaki
Sakakibara, Keiko
Tomita, Yuki
Togawa, Taisuke
Kunimoto, Kan
Osakabe, Akihisa
Suzuki, Yutaka
Yamato, Katsuyuki T.
Ishizaki, Kimitsune
Nishihama, Ryuichi
Kohchi, Takayuki
Franco-Zorrilla, José M.
Twell, David
Berger, Frédéric
Araki, Takashi
First Published: 11-Dec-2018
Publisher: Nature Research (part of Springer Nature)
Citation: Nature Communications, 2018, 9, 5283
Abstract: Evolutionary mechanisms underlying innovation of cell types have remained largely unclear. In multicellular eukaryotes, the evolutionary molecular origin of sperm differentiation is unknown in most lineages. Here, we report that in algal ancestors of land plants, changes in the DNA-binding domain of the ancestor of the MYB transcription factor DUO1 enabled the recognition of a new cis-regulatory element. This event led to the differentiation of motile sperm. After neo-functionalization, DUO1 acquired sperm lineage-specific expression in the common ancestor of land plants. Subsequently the downstream network of DUO1 was rewired leading to sperm with distinct morphologies. Conjugating green algae, a sister group of land plants, accumulated mutations in the DNA-binding domain of DUO1 and lost sperm differentiation. Our findings suggest that the emergence of DUO1 was the defining event in the evolution of sperm differentiation and the varied modes of sexual reproduction in the land plant lineage.
DOI Link: 10.1038/s41467-018-07728-3
eISSN: 2041-1723
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2018. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: Novel data generated in this study have been deposited at GenBank under the accessions: MpDUO1 (LC172177), MpR2R3-MYB07 (KX683859), MpR2R3-MYB21 (KX683860) MpACT1 (LC172182) MpCEN1 (LC379265) MpTUA5 (LC172181) MpDAZ1 (LC172178) MpGCS1 (LC172179) MpGEX2 (LC172180) HmnDUO1 (LC379264) HmnACT1 (LC379378) CbrDUO1 (LC199499), CauDUO1 (LC221833), CleDUO1 (LC221832), CbrRSP11 (LC382020) CbrPACRG (LC382019) CbrRPL6 (LC382018) CauRPL6 (LC382017) CpeDUO1 (LC176570). Previously reported sequences used in this study are available at GenBank under the accession: MpPACRG (LC102460), MpPRM (LC102462) MpTUB4 (KM096548) MpLC7 (LC102461), MpMID/RWP2 (KU987912) CleRPL6 (AB035569), CpeRLK1 (AB920609) CpePI (AB012698). All other data are available from the authors on reasonable request.
Appears in Collections:Published Articles, Dept. of Genetics

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