Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/43267
Title: A Novel Assay Format as an Alternative to ELISA: MINA Test for Biotin
Authors: Piletsky, SS
Cass, AEG
Piletska, E
Czulak, J
Piletsky, SA
First Published: 12-Sep-2018
Publisher: Wiley
Citation: ChemNanoMat 2018,Vol. 4, Issue 12, pp 1214–1222
Abstract: A novel abiotic assay based on biotin‐specific fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) which acted as both reporter probes and binding agents, was developed. This is a first report of an assay which, unlike ELISA, required no washing steps or addition of enzyme substrates, making it more user‐friendly. The components of the molecularly imprinted polymer nanoparticles assay (MINA) were assembled in microtiter plates fitted with magnetic inserts. The fluorescent nanoMIPs were bound to biotin‐conjugated magnetic particles, which were attracted to the inserts. The addition of free biotin caused a displacement of the fluorescent nanoMIPs into solution, generating a signal proportional to the concentration of biotin. The nanoMIPs had a dissociation constant (Kd) of 14 nM, allowing the assay to detect biotin at nano‐molar concentrations. The pre‐assembled assay only required the addition of the sample and measurement of the fluorescence, and it functioned well after six weeks of storage without refrigeration. The assay did not show the susceptibility to several compounds which are known to interfere with avidin and streptavidin‐based assays, such as mercaptoethanol and sugars. The protocols optimized in this work could be used to develop the abiotic assays for any other compound of interest.
DOI Link: 10.1002/cnma.201800393
eISSN: 2199-692X
Links: https://onlinelibrary.wiley.com/doi/full/10.1002/cnma.201800393
http://hdl.handle.net/2381/43267
Embargo on file until: 12-Sep-2019
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2018, Wiley. Deposited with reference to the publisher’s open access archiving policy. (http://www.rioxx.net/licenses/all-rights-reserved)
Description: The file associated with this record is under embargo until 12 months after publication, in accordance with the publisher's self-archiving policy. The full text may be available through the publisher links provided above.
Appears in Collections:Published Articles, Dept. of Chemistry

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