Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/4342
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dc.contributor.authorSong, Renfang-
dc.contributor.authorDuarte, Tiago L.-
dc.contributor.authorAlmeida, Gabriela M.-
dc.contributor.authorFarmer, Peter B.-
dc.contributor.authorCooke, Marcus S.-
dc.contributor.authorZhang, Wenbing-
dc.contributor.authorSheng, Guoying-
dc.contributor.authorFu, Jiamo-
dc.contributor.authorJones, George D. D.-
dc.date.accessioned2009-03-25T13:18:16Z-
dc.date.available2009-03-25T13:18:16Z-
dc.date.issued2008-11-27-
dc.identifier.citationToxicology Letters, 2009, 185 (1), pp. 23-31.en_GB
dc.identifier.issn0378-4274-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0378427408013556en_GB
dc.identifier.urihttp://hdl.handle.net/2381/4342-
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) are commonly used as flame retardants in a variety of commercial and household products. They have been detected in the environment and accumulate in mammalian tissues and fluids. PBDE toxicity is thought to be associated with endocrine disruption developmental neurotoxicity and changes in fetal development. Although humans are exposed to PBDEs, our knowledge of the effects of PBDE metabolites on human cells with respect to health risk is insufficient. Two hydroxylated PBDEs (OHPBDEs), 2-OH-BDE47 and 2-OH-BDE85, were investigated for their effects on cell viability/proliferation, DNA damage, cell cycle distribution and gene expression profiling in H295R adrenocortical carcinoma cells. We show that the two agents are cytotoxic in a dosedependent manner only at micromolar concentrations, with 2-OH-BDE85 being more toxic than 2-OH-BDE47. However, no DNA damage was observed for either chemical, suggesting that the biological effects of OH-PBDEs occur primarily via non-genotoxic routes. Furthermore, no evidence of aryl hydrocarbon receptor (AHR)-mediated, dioxin-like toxicity was observed. Instead, we report that a micromolar concentration of OH-PBDEs induces transcriptional changes associated with endoplasmic reticulum stress and the unfolded protein response. We discuss whether OH-PBDE bioaccumulation could result in impairment of the adrenocortical secretory function.en_GB
dc.language.isoenen_GB
dc.publisherElsevier.en_GB
dc.rightsThis is the author’s final draft of the paper published as Toxicology Letters, 2009, 185 (1), pp. 23-31. The final published version is available at http://www.elsevier.com, Doi: 10.1016/j.toxlet.2008.11.011.-
dc.subjectOH-PBDEen_GB
dc.subjectH295R adrenocortical carcinoma cellsen_GB
dc.subjecttoxicityen_GB
dc.subjectendoplasmic reticulum stressen_GB
dc.subjectunfolded protein responseen_GB
dc.titleCytotoxicity and gene expression profiling of two hydroxylated polybrominated diphenyl ethers in human H295R adrenocortical carcinoma cells.en_GB
dc.typeArticleen_GB
dc.identifier.doi10.1016/j.toxlet.2008.11.011-
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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