Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/44361
Title: Streptococcus pneumoniae Cell Wall-Localized Trigger Factor Elicits a Protective Immune Response and Contributes to Bacterial Adhesion to the Host.
Authors: Cohen, A
Troib, S
Dotan, S
Najmuldeen, H
Yesilkaya, H
Kushnir, T
Shagan, M
Portnoi, M
Nachmani, H
Benisty, R
Tal, M
Ellis, R
Chalifa-Caspi, V
Dagan, R
Nebenzahl, YM
First Published: 12-Mar-2019
Publisher: Nature Research (part of Springer Nature)
Citation: Scientific Reports, 2019, 9, Article number: 4295
Abstract: Trigger factor (TF) has a known cytoplasmic function as a chaperone. In a previous study we showed that pneumococcal TF is also cell-wall localized and this finding combined with the immunogenic characteristic of TF, has led us to determine the vaccine potential of TF and decipher its involvement in pneumococcal pathogenesis. Bioinformatic analysis revealed that TF is conserved among pneumococci and has no human homologue. Immunization of mice with recombinant (r)TF elicited a protective immune response against a pneumococcal challenge, suggesting that TF contributes to pneumococcal pathogenesis. Indeed, rTF and an anti-rTF antiserum inhibited bacterial adhesion to human lung derived epithelial cells, indicating that TF contributes to the bacterial adhesion to the host. Moreover, bacteria lacking TF demonstrated reduced adhesion, in vitro, to lung-derived epithelial cells, neural cells and glial cells. The reduced adhesion could be restored by chromosomal complementation. Furthermore, bacteria lacking TF demonstrated significantly reduced virulence in a mouse model. Taken together, the ability of rTF to elicit a protective immune response, involvement of TF in bacterial adhesion, conservation of the protein among pneumococcal strains and the lack of human homologue, all suggest that rTF can be considered as a future candidate vaccine with a much broader coverage as compared to the currently available pneumococcal vaccines.
DOI Link: 10.1038/s41598-019-40779-0
eISSN: 2045-2322
Links: https://www.nature.com/articles/s41598-019-40779-0
http://hdl.handle.net/2381/44361
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-40779-0.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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