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Title: Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal.
Authors: Ash, PA
Carr, SB
Reeve, HA
Skorupskaitė, A
Rowbotham, JS
Shutt, R
Frogley, MD
Evans, RM
Cinque, G
Armstrong, FA
Vincent, KA
First Published: 9-May-2017
Publisher: Royal Society of Chemistry
Citation: Chemical Communications, 2017, 53 (43), pp. 5858-5861
Abstract: We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchrotron infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electrochemical reduction from the oxidised inactive to the fully reduced state, and temporally resolve steps during this reduction.
DOI Link: 10.1039/c7cc02591b
eISSN: 1364-548X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: Electronic supplementary information (ESI) available: Crystal preparation; cyclic voltammogram in the experimental cell; position-dependent difference spectra; demonstration of control over all active sites; visible images of a crystal recorded periodically during measurements; time-dependent Nia-R formation. See DOI: 10.1039/c7cc02591b
Appears in Collections:Published Articles, Dept. of Chemistry

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