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Title: Blunted Cardiac AMPK Response is Associated with Susceptibility to Ischemia/Reperfusion in Male Offspring of Gestational Diabetic Rats.
Authors: Luo, X
Zou, H
Xu, P
Wen, L
Stanley, JL
Jiang, X
Han, T-L
Olson, D
Peng, C
Zhang, C
Xiao, X
Tong, C
Qi, H
Baker, PN
First Published: 13-Apr-2019
Publisher: Cell Physiol Biochem Press
Citation: Cellular Physiology and Biochemistry, 2019, 52 (5), pp. 1103-1116
Abstract: BACKGROUND/AIMS: Gestational diabetes mellitus (GDM) is closely associated with early perinatal complications and long-term health problems, such as cardiovascular disease, in offspring. AMP-activated protein kinase (AMPK) is cardioprotective, particularly in the treatment of ischemia/reperfusion (I/R). However, whether GDM programs offspring susceptibility to cardiac I/R and the involvement of AMPK remain unclear. METHODS: Streptozotocin was administered to rats during mid pregnancy; the postpartum maternal metabolome was assessed by chromatography-mass spectrometry (GC-MS). Male offspring were subjected to body composition scanning followed by ex vivo global I/R. Cardiac signaling was determined by Western blotting. RESULTS: The body weights (BWs) of the GDM male offspring were significantly heavier than those of the control group from the age of 8 weeks; the heart weights (HWs) and HW/BW were also increased in the GDM group compared to the control group. The ex vivo post-I/R cardiac contractile function recovery was significantly compromised in the GDM male offspring. The phosphorylation of AMPK and ACC was elevated by ex vivo I/R in both groups, but to a significantly lesser extent in the GDM group. CONCLUSION: GDM male offspring rats have higher risks of overgrowth and intolerance to cardiac I/R, which may be due to a compromised AMPK signaling pathway.
DOI Link: 10.33594/000000075
eISSN: 1421-9778
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License (, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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