Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/44567
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dc.contributor.authorEssid, Sumia Mohamed-
dc.contributor.authorBevington, Alan-
dc.contributor.authorBrunskill, Nigel J.-
dc.date.accessioned2019-06-24T10:48:25Z-
dc.date.available2019-06-24T10:48:25Z-
dc.date.issued2019-04-03-
dc.identifier.citationInternational Journal of Molecular Sciences, 2019, 20 (7)en
dc.identifier.urihttps://www.mdpi.com/1422-0067/20/7/1654en
dc.identifier.urihttp://hdl.handle.net/2381/44567-
dc.description.abstractThe repair capacity of progenitor skeletal muscle satellite cells (SC) in Type 1 diabetes mellitus (T1DM) is decreased. This is associated with the loss of skeletal muscle function. In T1DM, the deficiency of C-peptide along with insulin is associated with an impairment of skeletal muscle functions such as growth, and repair, and is thought to be an important contributor to increased morbidity and mortality. Recently, cholesterol-lowering drugs (statins) have also been reported to increase the risk of skeletal muscle dysfunction. We hypothesised that C-peptide activates key signaling pathways in myoblasts, thus promoting cell survival and protecting against simvastatin-induced myotoxicity. This was tested by investigating the effects of C-peptide on the L6 rat myoblast cell line under serum-starved conditions. Results: C-peptide at concentrations as low as 0.03 nM exerted stimulatory effects on intracellular signaling pathways-MAP kinase (ERK1/2) and Akt. When apoptosis was induced by simvastatin, 3 nM C-peptide potently suppressed the apoptotic effect through a pertussis toxin-sensitive pathway. Simvastatin strongly impaired Akt signaling and stimulated the reactive oxygen species (ROS) production; suggesting that Akt signaling and oxidative stress are important factors in statin-induced apoptosis in L6 myoblasts. The findings indicate that C-peptide exerts an important protective effect against death signaling in myoblasts. Therefore, in T1DM, the deficiency of C-peptide may contribute to myopathy by rendering myoblast-like progenitor cells (involved in muscle regeneration) more susceptible to the toxic effects of insults such as simvastatin.en
dc.description.sponsorshipThis research was supported by the Ministry of Higher Education and Scientific Research, Libya.en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/30987105-
dc.rightsCopyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectType 1 diabetesen
dc.subjectmyotoxicityen
dc.subjectproinsulin C-peptideen
dc.subjectsimvastatinen
dc.titleProinsulin C-Peptide Enhances Cell Survival and Protects against Simvastatin-Induced Myotoxicity in L6 Rat Myoblasts.en
dc.typeJournal Articleen
dc.identifier.doi10.3390/ijms20071654-
dc.identifier.eissn1422-0067-
dc.identifier.piiijms20071654-
dc.description.statusPeer-revieweden
dc.description.versionPublisher Versionen
dc.type.subtypeJournal Article-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF LIFE SCIENCESen
pubs.organisational-group/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciencesen
pubs.organisational-group/Organisation/COLLEGE OF LIFE SCIENCES/Themesen
pubs.organisational-group/Organisation/COLLEGE OF LIFE SCIENCES/Themes/Cardiovascularen
pubs.organisational-group/Organisation/COLLEGE OF LIFE SCIENCES/Themes/Molecular & Cellular Bioscienceen
dc.dateaccepted2019-03-25-
Appears in Collections:Published Articles, Dept. of Biology

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