Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/44576
Title: Peptidergic signaling from clock neurons regulates reproductive dormancy in Drosophila melanogaster.
Authors: Nagy, D
Cusumano, P
Andreatta, G
Anduaga, AM
Hermann-Luibl, C
Reinhard, N
Gesto, J
Wegener, C
Mazzotta, G
Rosato, E
Kyriacou, CP
Helfrich-Förster, C
Costa, R
First Published: 13-Jun-2019
Publisher: Public Library of Science
Citation: PLoS Genetics, 2019, 15(6): e1008158.
Abstract: With the approach of winter, many insects switch to an alternative protective developmental program called diapause. Drosophila melanogaster females overwinter as adults by inducing a reproductive arrest that is characterized by inhibition of ovarian development at previtellogenic stages. The insulin producing cells (IPCs) are key regulators of this process, since they produce and release insulin-like peptides that act as diapause-antagonizing hormones. Here we show that in D. melanogaster two neuropeptides, Pigment Dispersing Factor (PDF) and short Neuropeptide F (sNPF) inhibit reproductive arrest, likely through modulation of the IPCs. In particular, genetic manipulations of the PDF-expressing neurons, which include the sNPF-producing small ventral Lateral Neurons (s-LNvs), modulated the levels of reproductive dormancy, suggesting the involvement of both neuropeptides. We expressed a genetically encoded cAMP sensor in the IPCs and challenged brain explants with synthetic PDF and sNPF. Bath applications of both neuropeptides increased cAMP levels in the IPCs, even more so when they were applied together, suggesting a synergistic effect. Bath application of sNPF additionally increased Ca2+ levels in the IPCs. Our results indicate that PDF and sNPF inhibit reproductive dormancy by maintaining the IPCs in an active state.
DOI Link: 10.1371/journal.pgen.1008158
eISSN: 1553-7404
Links: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008158
http://hdl.handle.net/2381/44576
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: Uncorrected proof.
Appears in Collections:Published Articles, Dept. of Genetics

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