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Title: Sputum microbiome temporal variability and dysbiosis in chronic obstructive pulmonary disease exacerbations: an analysis of the COPDMAP study.
Authors: Wang, Z
Singh, R
Miller, BE
Tal-Singer, R
Van Horn, S
Tomsho, L
Mackay, A
Allinson, JP
Webb, AJ
Brookes, AJ
George, LM
Barker, B
Kolsum, U
Donnelly, LE
Belchamber, K
Barnes, PJ
Singh, D
Brightling, CE
Donaldson, GC
Wedzicha, JA
Brown, JR
First Published: 21-Dec-2017
Publisher: BMJ Publishing Group with British Thoracic Society (BTS)
Citation: Thorax, 2018, 73 (4), pp. 331-338
Abstract: BACKGROUND: Recent studies suggest that lung microbiome dysbiosis, the disease associated disruption of the lung microbial community, might play a key role in chronic obstructive pulmonary disease (COPD) exacerbations. However, characterising temporal variability of the microbiome from large longitudinal COPD cohorts is needed to better understand this phenomenon. METHODS: We performed a 16S ribosomal RNA survey of microbiome on 716 sputum samples collected longitudinally at baseline and exacerbations from 281 subjects with COPD at three UK clinical centres as part of the COPDMAP consortium. RESULTS: The microbiome composition was similar among centres and between stable and exacerbations except for a small significant decrease of Veillonella at exacerbations. The abundance of Moraxella was negatively associated with bacterial alpha diversity. Microbiomes were distinct between exacerbations associated with bacteria versus eosinophilic airway inflammation. Dysbiosis at exacerbations, measured as significant within subject deviation of microbial composition relative to baseline, was present in 41% of exacerbations. Dysbiosis was associated with increased exacerbation severity indicated by a greater fall in forced expiratory volume in one second, forced vital capacity and a greater increase in CAT score, particularly in exacerbations with concurrent eosinophilic inflammation. There was a significant difference of temporal variability of microbial alpha and beta diversity among centres. The variation of beta diversity significantly decreased in those subjects with frequent historical exacerbations. CONCLUSIONS: Microbial dysbiosis is a feature of some exacerbations and its presence, especially in concert with eosinophilic inflammation, is associated with more severe exacerbations indicated by a greater fall in lung function. TRIAL REGISTRATION NUMBER: Results, NCT01620645.
DOI Link: 10.1136/thoraxjnl-2017-210741
eISSN: 1468-3296
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2017, BMJ Publishing Group with British Thoracic Society (BTS). Deposited with reference to the publisher’s open access archiving policy. (
Appears in Collections:Published Articles, Dept. of Genetics

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COPDMAP Supplementary Files_V16.docxPost-review (final submitted author manuscript)1.26 MBUnknownView/Open
COPDMAP Manuscript V25_clean.pdfPost-review (final submitted author manuscript)457.82 kBAdobe PDFView/Open

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