Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/44829
Title: A variant NuRD complex containing PWWP2A/B excludes MBD2/3 to regulate transcription at active genes.
Authors: Zhang, T
Wei, G
Millard, CJ
Fischer, R
Konietzny, R
Kessler, BM
Schwabe, JWR
Brockdorff, N
First Published: 18-Sep-2018
Publisher: Springer Nature
Citation: Nature Communications, 2018, 9 (1), p. 3798
Abstract: Transcriptional regulation by chromatin is a highly dynamic process directed through the recruitment and coordinated action of epigenetic modifiers and readers of these modifications. Using an unbiased proteomic approach to find interactors of H3K36me3, a modification enriched on active chromatin, here we identify PWWP2A and HDAC2 among the top interactors. PWWP2A and its paralog PWWP2B form a stable complex with NuRD subunits MTA1/2/3:HDAC1/2:RBBP4/7, but not with MBD2/3, p66α/β, and CHD3/4. PWWP2A competes with MBD3 for binding to MTA1, thus defining a new variant NuRD complex that is mutually exclusive with the MBD2/3 containing NuRD. In mESCs, PWWP2A/B is most enriched at highly transcribed genes. Loss of PWWP2A/B leads to increases in histone acetylation predominantly at highly expressed genes, accompanied by decreases in Pol II elongation. Collectively, these findings suggest a role for PWWP2A/B in regulating transcription through the fine-tuning of histone acetylation dynamics at actively transcribed genes.
DOI Link: 10.1038/s41467-018-06235-9
eISSN: 2041-1723
Links: http://hdl.handle.net/2381/44829
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2018. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: All the high-throughput data (ChIP-seq, 4sU-RNA-seq) generated in this study have been deposited in GEO under GSE112114. Mass spectrometry data are available via ProteomeXchange with identifier PXD010445.
Appears in Collections:Published Articles, Dept. of Molecular and Cell Biology

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