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Title: New methods for assessing male germline mutations in humans and genetic risks in their offspring.
Authors: Verhofstad, Nicole
Linschooten, Joost O.
van Benthem, Jan
Dubrova, Yuri E.
van Steeg, Harry
van Schooten, Frederik J.
Godschalk, Roger W. L.
First Published: 20-May-2008
Publisher: Oxford University Press
Citation: Mutagenesis, 2008, 23 (4), pp. 241-247
Abstract: Germline mutations resulting from chemical or radiation exposure are a particular problem in toxicology as they affect not only the exposed generation, but an infinite number of generations thereafter. Established methods to show that these mutations occur in an F1 or subsequent population require the use of a large number of progeny for statistical significance. Consequently, many thousands of animals have been used in the past. Such a use is no longer considered desirable and is also very expensive. Several new molecular techniques (including analysis of tandem repeats and randomly amplified polymorphic DNA) now provide alternative methods of assessment, which also allow the quantification of individual mutations in individual sperm cells. These can also be applied to human offspring, making extrapolation obsolete. The downside of these methods is that they effectively determine the mutation rate in certain regions of DNA and the relevance of these to diseases, particularly cancer, is not always apparent. Therefore it must be assumed that an increase in mutation rates in these selected regions correlates with altered phenotype. However, disease types linked to changes in tandem repeat length indicate that these may act as relevant markers for the development of phenotypes. Further research and evaluation is required to more closely link changes in DNA with altered phenotype and validate the use of tandem repeats and randomly amplified polymorphic DNA in transgenerational genotoxicity testing. This paper introduces and compares recently developed methods to assess mutations in sperm due to stem cell damage.
DOI Link: 10.1093/mutage/gen022
ISSN: 0267-8357
Type: Article
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version, Mutagenesis, 2008, 23 (4), pp. 241-247, is available online at: Doi: 10.1093/mutage/gen022
Appears in Collections:Published Articles, Dept. of Genetics

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