Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/45189
Title: Sputum Moraxella catarrhalis strains exhibit diversity within and between COPD subjects.
Authors: George, LM
Haigh, RD
Mistry, V
Haldar, K
Barer, MR
Oggioni, MR
Brightling, CE
First Published: 8-Nov-2018
Publisher: Dove Medical Press
Citation: International Journal of Chronic Obstructive Pulmonary Disease, 2018, 13, pp. 3663-3667
Abstract: Purpose: Moraxella catarrhalis is implicated in the pathogenesis of some COPD exacerbations. We sought to investigate whether the M. catarrhalis strain is variable between COPD subjects; that an exacerbation is associated with acquisition of a new strain and that certain strains are more commonly associated with exacerbations. Patients and methods: Sputum samples were collected at stable and exacerbation visits from COPD subjects from a single center as part of the COPDMAP consortium. Samples identified as M. catarrhalis positive by qPCR were recultured in liquid cultures grown to extract genomic DNA; underwent Illumina MiSeq and bacterial genome sequences were de novo assembled and Multi Locus Sequence Type (MLST) was determined. Results: Thirty-five samples were obtained from 18 subjects. These included 13 stable and 22 exacerbation samples. The diversity between samples was very large with 25 different M. catarrhalis MLSTs being identified out of the 35 samples of which 12 MSLTs have not been described previously. Change and persistence of M. catarrhalis strain were observed between stable visits, from stable to exacerbation and vice-a-versa, and between exacerbation visits. Conclusion: Sputum M. catarrhalis strains exhibit marked diversity within and between COPD subjects. Acquisition of a new strain is common between stable and exacerbation events such that no strain is specifically associated with an exacerbation.
DOI Link: 10.2147/COPD.S180961
eISSN: 1178-2005
Links: https://www.dovepress.com/sputum-moraxella-catarrhalis-strains-exhibit-diversity-within-and-betw-peer-reviewed-article-COPD
http://hdl.handle.net/2381/45189
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2018. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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