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Title: Functional assessment of microbial superoxide dismutase isozymes suggests a differential role for each isozyme.
Authors: Najmuldeen, Hastyar
Alghamdi, Rashed
Alghofaili, Fayez
Yesilkaya, Hasan
First Published: 15-Jan-2019
Publisher: Elsevier
Citation: Free Radical Biology and Medicine, 2019, 134, pp. 215-228
Abstract: Microbes can have multiple enzymes that are able to catalyse the same enzymatic reactions but may differ in structure. These are known as isozymes. It is assumed that isozymes have the same functional role for cells. Contrary to this assumption, we hypothesised that isozymes can confer different functions for microbial cells despite catalysing the same reactions. To test this hypothesis, we studied the role of superoxide dismutases (SOD) in Klebsiella pneumoniae, the causative agent of several nosocomial and community-acquired infections, in infection relevant assays. SODs are responsible for detoxification of toxic superoxide radicals. K. pneumoniae genome contains three superoxide dismutase genes, sodA, sodB, and sodC coding for Mn-, Fe- and CuZn- co-factored SODs, respectively. By creating and testing single, double, and triple SOD mutants, we investigated the regulatory interactions among SOD and determined the role of each isozyme in oxidative stress resistance, biofilm formation, cell morphology, metabolism, and in vivo colonization and persistence. Our results demonstrate that SOD isozymes in K. pneumoniae have unique roles beyond oxidative stress resistance, and there is a regulatory interplay among SODs.
DOI Link: 10.1016/j.freeradbiomed.2019.01.018
eISSN: 1873-4596
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License (, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Description: Supplementary data associated with this article can be found in the online version at doi:10.1016/j.freeradbiomed.2019.01.018.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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