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Title: Locomotor Behaviour and Clock Neurons Organisation in the Agricultural Pest Drosophila suzukii.
Authors: Hansen, Celia Napier
Özkaya, Özge
Roe, Helen
Kyriacou, Charalambos P.
Giongo, Lara
Rosato, Ezio
First Published: 24-Jul-2019
Publisher: Frontiers Media
Citation: Frontiers in Physiology, 2019, 10:941
Abstract: Drosophila suzukii (Matsumara) also called Spotted Wing Drosophila (SWD), is an invasive pest species originally from Asia that has now spread widely across Europe and North America. The majority of drosophilids including the best known Drosophila melanogaster only breed on decaying fruits. On the contrary, the presence of a strong serrated ovipositor and behavioural and metabolic adaptations allow D. suzukii to lay eggs inside healthy, ripening fruits that are still on the plant. Here we present an analysis of the rhythmic locomotor activity behaviour of D. suzukii under several laboratory settings. Moreover, we identify the canonical clock neurons in this species by reporting the expression pattern of the major clock proteins in the brain. Interestingly, a fundamentally similar organisation of the clock neurons network between D. melanogaster and D. suzukii does not correspond to similar characteristics in rhythmic locomotor activity behaviour.
DOI Link: 10.3389/fphys.2019.00941
ISSN: 1664-042X
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © the authors, 2019. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Description: The Supplementary Material for this article can be found online at: Figure S1. Average day profile for D. suzukii males S1210, SMichele, S1203, and S1209 under standard laboratory conditions. The average day was obtained by averaging into one the four LD days for all rhythmic flies (SR+CR, see Table 1) of the same genotype. Black columns correspond to dark and white columns to light. Error bars show the standard deviation. Note that the error bars reaching the top of the graph are truncated. This reflects a compromise in the choice of the scale of the Y-axis to allow the appreciation of both the average activity profile and its variance. Activity levels (0–40 crossing/30 min) are shown on the Y-axis, time (48 intervals of 30 min) is on the X-axis. Note that the profile for S1210 is the same as in Figure 1B but at different scale on the Y-axis. Figure S2. Combined LD activity under all entrainment conditions in D. melanogaster (M1217) and D. suzukii (S1202) virgin females (fv) and males (m). To test whether virgin females and males from the two species differ in their average activity under LD we combined 31 flies (the maximum common number of rhythmic flies across conditions, genders and species) at random for each entrainment condition. In M1217, virgin females (mean LD activity = 18.21 crossing/30 min) were more active than males (mean LD activity = 16.88 crossing/30 min); the difference is statistically supported (Kolmogorov-Smirnov test, ***P = 0.0003). In S1202, virgin females (mean LD activity = 3.55 crossing/30 min) and males (mean LD activity = 3.61 crossing/30 min) showed equal activity. Note that D. melanogaster flies are about five times more active than D. suzukii. Figure S3. Population rhythms. Activity levels in 10 males D. suzukii (S1202) and 10 males D. melanogaster (M1217). Activity levels (number of crossing/30 min) have been plotted separately for the three detector rings (R1, R2, and R3) to give an impression of the overall activity. See also Table S1. Figure S4. Anti-PDF staining of the LNv in D. suzukii and D. melanogaster. Typically, in D. suzukii the l-LNv are closer to one another and to the s-LNv than in D. melanogaster. L = l-LNv. s = s-LNv. Each panel represents confocal maximum projections of several z-stacks. Contrast and brightness have been optimised, modifications were applied to each panel as a whole. Table S1. Descriptive statistics for amount of activity/bin. The locomotor activities of 10 D. suzukii males and 10 D. melanogaster males were recorded for 5 days under LD 12:12, 25°C. Video 1. A simple startling stimulus, banging a growth vial on the bench, is applied to D. suzukii and D. melanogaster cultures. The locomotor response of the two species is different showing high arousal and activity in D. melanogaster and reduced arousal and activity in D. suzukii.
Appears in Collections:Published Articles, Dept. of Genetics

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