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Title: A split-protein sensor for studying protein–protein interaction in mycobacteria
Authors: O'Hare, Helen M.
Juillerat, Alexandre
Dianišková, Petronela
Johnsson, Kai
First Published: May-2008
Publisher: Elsevier
Citation: Journal of Microbiological Methods, 2008, 73 (2), pp. 79-84.
Abstract: The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a split-protein sensor used to detect protein–protein interactions in tryptophan auxotrophic Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to Escherichia coli and Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms.
ISSN: 0167-7012
Type: Article
Description: This paper was published as Journal of Microbiological Methods, 2008, 73 (2), pp. 79-84. It is available from Doi: 10.1016/j.mimet.2008.02.008
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Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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