Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/48
Title: Proximal C-terminal domain of sulphonylurea receptor 2A interacts with pore-forming Kir6 subunits in KATP channels
Authors: Rainbow, Richard Daniel
James, Marian
Hudman, Diane
Al Johi, Mohammed
Singh, Harprit
Watson, Peter J.
Ashmole, Ian
Davies, Noel W.
Lodwick, David
Norman, Robert I.
First Published: 1-May-2004
Publisher: Biochemical Society
Citation: Biochemical Journal, 2004, 379, pp173-181
Abstract: Functional KATP (ATP-sensitive potassium) channels are heterooctamers of four Kir6 (inwardly rectifying potassium) channel subunits and four SUR (sulphonylurea receptor) subunits. Possible interactions between the C-terminal domain of SUR2A and Kir6.2 were investigated by co-immunoprecipitation of rat SUR2A C-terminal fragments with full-length Kir6.2 and by analysis of cloned KATP channel function and distribution in HEK-293 cells (human embryonic kidney 293 cells) in the presence of competing rSUR2A fragments. Three maltose-binding protein–SUR2A fusions, rSUR2A-CTA (rSUR2A residues 1254–1545), rSUR2A-CTB (residues 1254–1403) and rSUR2A-CTC (residues 1294–1403), were co-immunoprecipitated with full-length Kir6.2 using a polyclonal anti-Kir6.2 antiserum. A fourth C-terminal domain fragment, rSUR2A-CTD (residues 1358–1545) did not co-immunoprecipitate with Kir6.2 under the same conditions, indicating a direct interaction between Kir6.2 and a 65-amino-acid section of the cytoplasmic C-terminal region of rSUR2A between residues 1294 and 1358. ATP- and glibenclamide-sensitive K+ currents were decreased in HEK-293 cells expressing full-length Kir6 and SUR2 subunits that were transiently transfected with fragments rSUR2A-CTA, rSUR2A-CTC and rSUR2A-CTE (residues 1294–1359) compared with fragment rSUR2A-CTD or mock-transfected cells, suggesting either channel inhibition or a reduction in the number of functional KATP channels at the cell surface. Anti-KATP channel subunit-associated fluorescence in the cell membrane was substantially lower and intracellular fluorescence increased in rSUR2A-CTE expressing cells; thus, SUR2A fragments containing residues 1294–1358 reduce current by decreasing the number of channel subunits in the cell membrane. These results identify a site in the C-terminal domain of rSUR2A, between residues 1294 and 1358, whose direct interaction with full-length Kir6.2 is crucial for the assembly of functional KATP channels.
ISSN: 0264-6021
eISSN: 1470-8728
Links: http://hdl.handle.net/2381/48
http://www.biochemj.org/bj/379/bj3790173.htm
Version: Publisher version
Status: Peer reviewed
Type: Article
Rights: Copyright © 2004 Biochemical Society.
Description: Mounted on the Internet with the permission of The Biochemical Society (2004). Also available from http://www.biochemj.org/bj/379/bj3790173.htm
Appears in Collections:Published Articles, Dept. of Cardiovascular Sciences

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