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|Title:||Click chemistry as a reliable method for the high-density functionalisation of alkyne-modified oligodeoxyribonucleotides.|
Burley, G. A.
Gramlich, P. M. E.
Hammond, D. M.
|Citation:||Organic Letters, 2006, 8 (17), pp.3639-3642|
|Abstract:||We report the development of the Cu(I)-catalyzed Huisgen cycloaddition (click) reaction for the multiple postsynthetic labeling of alkyne-modified DNA. A series of alkyne-modified oligodeoxyribonucleotides (ODNs) of increasing alkyne density were prepared, and the click reaction using various azide labels was investigated. Complete high-density conversion was observed for ODNs containing up to six consecutive alkyne functions. Compatibility of the click conditions with long DNA strands was shown using a PCR product obtained with an alkyne-modified primer.|
|Appears in Collections:||Published Articles, Dept. of Chemistry|
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