Please use this identifier to cite or link to this item:
Title: Click chemistry as a reliable method for the high-density functionalisation of alkyne-modified oligodeoxyribonucleotides.
Authors: Gierlich, J.
Burley, G. A.
Gramlich, P. M. E.
Hammond, D. M.
Carell, T.
First Published: 2006
Citation: Organic Letters, 2006, 8 (17), pp.3639-3642
Abstract: We report the development of the Cu(I)-catalyzed Huisgen cycloaddition (click) reaction for the multiple postsynthetic labeling of alkyne-modified DNA. A series of alkyne-modified oligodeoxyribonucleotides (ODNs) of increasing alkyne density were prepared, and the click reaction using various azide labels was investigated. Complete high-density conversion was observed for ODNs containing up to six consecutive alkyne functions. Compatibility of the click conditions with long DNA strands was shown using a PCR product obtained with an alkyne-modified primer.
DOI Link: 10.1021/ol0610946
ISSN: 1523-7060
Type: Article
Appears in Collections:Published Articles, Dept. of Chemistry

Files in This Item:
There are no files associated with this item.

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.