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|Title:||Crystal structure of the ascorbate peroxidase-ascorbate complex.|
Moody, Peter C.E.
|Citation:||Nature Structural Biology, 2003, 10 (4), pp.303-307|
|Abstract:||Heme peroxidases catalyze the H2O2-dependent oxidation of a variety of substrates, most of which are organic. Mechanistically, these enzymes are well characterized: they share a common catalytic cycle that involves formation of a two-electron, oxidized Compound I intermediate followed by two single-electron reduction steps by substrate. The substrate specificity is more diverse — most peroxidases oxidize small organic substrates, but there are prominent exceptions — and there is a notable absence of structural information for a representative peroxidase−substrate complex. Thus, the features that control substrate specificity remain undefined. We present the structure of the complex of ascorbate peroxidase−ascorbate. The structure defines the ascorbate-binding interaction for the first time and provides new rationalization of the unusual functional features of the related cytochrome c peroxidase enzyme, which has been a benchmark for peroxidase catalysis for more than 20 years. A new mechanism for electron transfer is proposed that challenges existing views of substrate oxidation in other peroxidases.|
|Appears in Collections:||Published Articles, Dept. of Chemistry|
Published Articles, Dept. of Biochemistry
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