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Title: Inter-laboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine.
Authors: Cooke, Marcus S.
Barregard, Lars
Mistry, Vilas
Potdar, Neelam
Rozalski, Rafal
Gackowski, Daniel
Siomek, Agnieszka
Foksinski, Marek
Svoboda, Peter
Kasai, Hiroshi
Konje, Justin C.
Sallsten, Gerd
Evans, Mark D.
Olinski, Ryszard
First Published: Mar-2009
Publisher: Taylor & Francis
Citation: Biomarkers, 2009, 14 (2), pp. 103-110.
Abstract: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg-1 creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.
DOI Link: 10.1080/13547500802706012
ISSN: 1354-750X
Type: Article
Rights: This is the author's final draft of the paper published as Biomarkers, 2009, 14 (2), pp. 103-110. The final version is available from Doi: 10.1080/13547500802706012
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine
Published Articles, Dept. of Genetics

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