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|Title:||Inter-laboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine.|
|Authors:||Cooke, Marcus S.|
Konje, Justin C.
Evans, Mark D.
|Publisher:||Taylor & Francis|
|Citation:||Biomarkers, 2009, 14 (2), pp. 103-110.|
|Abstract:||Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg-1 creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.|
|Description:||This is the author's final draft of the paper published as Biomarkers, 2009, 14 (2), pp. 103-110. The final version is available from http://www.informaworld.com. Doi: 10.1080/13547500802706012|
|Appears in Collections:||Published Articles, Dept. of Cancer Studies and Molecular Medicine|
Published Articles, Dept. of Genetics
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