Leicester Research Archive

Leicester Research Archive >
College of Medicine, Biological Sciences and Psychology >
Cell Physiology and Pharmacology, Department of >
Published Articles, Dept. of Cell Physiology and Pharmacology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/8061

Title: Identification of cAMP-Dependent Kinase as a Third in Vivo Ribosomal Protein S6 Kinase in Pancreatic β-Cells
Authors: Moore, Claire E.J.
Xie, Jianling
Gomez, Edith
Herbert, Terence P.
Issue Date: 16-Apr-2009
Publisher: Elsevier
Citation: Journal of Molecular Biology, 2009, 389 (3), pp. 480-494
Abstract: Ribosomal protein S6 (rpS6) is phosphorylated in vivo by isoforms of p70 S6 protein kinase and p90 ribosomal S6 kinase, and there is good evidence that it plays a positive role in controlling pancreatic β-cell size and function. In this report, we demonstrate in the pancreatic β-cell line MIN6 (mouse insulinoma cell line 6) and islets of Langerhans that agents which stimulate increases in cAMP, such as glucagon-like peptide-1 and forskolin, lead to the phosphorylation of rpS6 at Ser235/Ser236 independently of the activation of the currently known in vivo rpS6 kinases via a pathway that is sensitive to inhibitors of cAMP-dependent protein kinase [protein kinase A (PKA)]. This cAMP-dependent rpS6 kinase activity is also sensitive to PKI in vitro, and PKA exclusively phosphorylates recombinant rpS6 on Ser235/Ser236 in vitro. With these data taken together, we conclude that PKA can phosphorylate rpS6 exclusively at Ser235/Ser236 in vivo in pancreatic β-cells, thus providing a potentially important link between cAMP signalling and the regulation of protein synthesis. Lastly, we provide evidence that PKA is also likely to phosphorylate rpS6 on Ser235/Ser236 in vivo in a number of other mammalian cell types.
ISSN: 0022-2836
Links: http://www.sciencedirect.com/science/journal&(...)
http://dx.doi.org/10.1016/j.jmb.2009.04.020
http://hdl.handle.net/2381/8061
Type: Article
Description: This is the author’s final draft of the paper published as Journal of Molecular Biology, 2009, 389 (3), pp. 480-494. The final published version is available at http://www.sciencedirect.com/science/journal/00222836, Doi: 10.1016/j.jmb.2009.04.020
Appears in Collections:Published Articles, Dept. of Cell Physiology and Pharmacology

Files in This Item:

File Description SizeFormat
[02]Moore Xie et al 150209 .pdf1.42 MBAdobe PDFView/Open
View Statistics

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

MAINTAINER