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Title: Diagnostic Testing of Patients Suspected of Primary Ciliary Dyskinesia
Authors: Stannard, Wendy A.
Chilvers, Mark A.
Rutman, Andrew R.
Williams, Chris D.
O'Callaghan, Christopher L.
First Published: 12-Nov-2009
Publisher: American Thoracic Society
Citation: American Journal of Respiratory and Critical Care Medicine, 2010, 181 (4), pp. 307-314
Abstract: Rationale: Electron microscopy (EM) of ciliated epithelium is widely used to diagnose primary ciliary dyskinesia (PCD). Ciliary beat frequency (CBF) has been used to screen samples to determine whether EM is indicated. Beat pattern analysis has been advocated as an additional diagnostic test. Neither has been subject to formal review. Objectives: To determine the ability of CBF and beat pattern analysis to predict EM-diagnosed PCD. Methods: CBF calculation and beat pattern analysis, using high-speed video microscopy, and EM were performed on nasal tissue from 371 patients consecutively referred to the Leicester Royal Infirmary for diagnostic assessment for PCD. With EM as the "gold standard," receiver operating characteristic (ROC) curves were constructed and sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values were calculated for CBF less than 11 Hz, ciliary dyskinesia score equal to or exceeding 2, at least 90% of ciliated edges beating dyskinetically, and an immotility index equal to or exceeding 10%. Measurements and Main Results: PCD was excluded in 270 patients and confirmed in 70 by EM. The sensitivity, specificity, PPV, and NPV for CBF less than 11 Hz were 87.1, 77.2, 50.0, and 95.8%, respectively. These values were higher for ciliary dyskinesia scores equal to or exceeding 2 (92.5, 97.6, 91.2, and 98.0%) and when at least 90% of ciliated edges were dyskinetic (97.1, 95.3, 84.6, and 99.2%). ROCs confirmed that the ciliary dyskinesia score and percentage of dyskinetic edges were superior screening indices compared with CBF and the immotility index. Conclusions: The use of CBF alone to screen which biopsies should have EM will result in a significant number of missed diagnoses. Ciliary beat pattern analysis is a more sensitive and specific test for PCD with higher PPV and NPV.
ISSN: 1073-449X
Type: Article
Description: Full text of this item is not currently available on the LRA. The final published version is available at, Doi: 10.1164/rccm.200903-0459OC.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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