Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/8772
Title: Small fitness effects and weak genetic interactions between deleterious mutations in heterozygous loci of the yeast Saccharomyces cerevisiae
Authors: Szafraniec, Krzysztof
Wloch, Dominika M.
Sliwa, Piotr
Borts, Rhona H.
Korona, Ryszard
First Published: 10-Sep-2003
Publisher: Cambridge University Press
Citation: Genetical Research (2003), 82(1), pp. 19-31.
Abstract: Rare, random mutations were induced in budding yeast by ethyl methanesulfonate (EMS). Clones known to bear a single non-neutral mutation were used to obtain mutant heterozygotes and mutant homozygotes that were later compared with wild-type homozygotes. The average homozygous effect of mutation was an approximately 2% decrease in the growth rate. In heterozygotes, the harmful effect of these relatively mild mutations was reduced approximately fivefold. In a test of epistasis, two heterozygous mutant loci were paired at random. Fitness of the double mutants was best explained by multiplicative action of effects at single loci, with little evidence for epistasis and essentially excluding synergism. In other experiments, the same mutations in haploid and heterozygous diploid clones were compared. Regardless of the haploid phenotypes, mildly deleterious or lethal, fitness of the heterozygotes was decreased by less than half a per cent on average. In general, the results presented here suggest that most mutations tend to exhibit small and weakly interacting effects in heterozygous loci regardless of how harmful they are in haploids or homozygotes.
DOI Link: 10.1017/S001667230300630X
ISSN: 0016-6723 (print)
1469-5073 (electronic)
Links: http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=175907&fileId=S001667230300630X
http://hdl.handle.net/2381/8772
Type: Article
Rights: This is the publisher's PDF of the article originally published as Genetical Research (2003), 82(1), pp. 19-31, also available on the publisher's website at: http://journals.cambridge.org/action/displayBackIssues?jid=GRH doi: 10.1017/S001667230300630X
Appears in Collections:Published Articles, Dept. of Genetics

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