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|Title:||Mobile D-loops are a preferred substrate for the Bloom’s syndrome helicase|
|Authors:||Bachrati, Csanád Z.|
Borts, Rhona H.
Hickson, Ian D.
|Publisher:||Oxford University Press|
|Citation:||Nucleic Acids Research, 2006, 34(8), pp. 2269-2279.|
|Abstract:||The Bloom’s syndrome helicase, BLM, is a member of the highly conserved RecQ family, and possesses both DNA unwinding and DNA strand annealing activities. BLM also promotes branch migration of Holliday junctions. One role for BLM is to act in conjunction with topoisomerase IIIa to process homologous recombination (HR) intermediates containing a double Holliday junction by a process termed dissolution. However, several lines of evidence suggest that BLM may also act early in one or more of the recombination pathways to eliminate illegitimate or aberrantly paired DNA joint molecules. We have investigated whether BLM can disrupt DNA displacement loops (D-loops), which represent the initial strand invasion step of HR. We show that mobile D-loops created by the RecA recombinase are a highly preferred substrate for BLM with the invading strand being displaced from the duplex. We have identified structural features of the D-loop that determine the efficiency with which BLM promotes D-loop dissociation. We discuss these results in the context of models for the role of BLM as an ‘anti-recombinase’.|
|Rights:||This is the publisher-produced PDF of the article published as Nucleic Acids Research, 2006, 34(8), pp. 2269-2279. The original published article is available on open access on the publisher's website at http://nar.oxfordjournals.org/ doi: 10.1093/nar/gkl258|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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