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dc.contributor.authorHirst, Robert A.-
dc.contributor.authorGosai, Bejal-
dc.contributor.authorRutman, Andrew-
dc.contributor.authorGuerin, Christopher J.-
dc.contributor.authorNicotera, Pierluigi-
dc.contributor.authorAndrew, Peter W.-
dc.contributor.authorO'Callaghan, Christopher L.-
dc.identifier.citationJournal of Infectious Diseases, 2008, 197 (5), pp. 744-751en_GB
dc.description.abstractBackground: The role played by pneumolysin and autolysin in pneumococcal meningitis is poorly understood. Method: A rat model was used to investigate the disease, in which surgical implantation of a cisternal catheter allowed bacterial instillation and cerebrospinal fluid (CSF) sampling. Results: CSF infection of rats with wild-type pneumococci caused meningitis within 26 h, whereas isogenic mutants that do not express pneumolysin (∆Ply) or autolysin (LytA ¯) caused very mild or no disease. Wild-type infections resulted in pneumococci in the CSF and cortical homogenates, but a minority of the rats infected with ∆Ply or LytA ¯had bacteria in these locations at 26 h. Leukocyte numbers in the CSF were similar after infection with all pneumococci; however, neutrophils and monocytes predominated after wild-type infection, whereas lymphocytes and atypical lymphocytes predominated after infection with the mutants. Wild-type pneumococci caused disruption to the ependyma, but this was not observed in rats infected with ∆Ply or LytA ¯. Cells surrounding the ventricles in wild type–infected animals expressed caspase 3, and astrocytes had hypertrophy; both findings were absent in rats infected with the mutants. Conclusions: This study provides strong in vivo evidence that pneumolysin and autolysin play crucial roles in the pathogenesis of pneumococcal meningitis.en_GB
dc.publisherUniversity of Chicago Pressen_GB
dc.rightsCopyright © 2010. University of Chicago Press. All rights reserved.-
dc.rightsThis is the final publisher edited version of the paper published as Journal of Infectious Diseases, 2008, 197 (5), pp. 744-751. This version was first published at, Doi: 10.1086/527322.-
dc.titleStreptococcus pneumoniae Deficient in Pneumolysin or Autolysin Has Reduced Virulence in Meningitisen_GB
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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