Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/8863
Title: Ciliary dysfunction and ultrastructural abnormalities are features of severe asthma
Authors: Thomas, Biju
Rutman, Andrew
Hirst, Robert A.
Haldar, Pranab
Wardlaw, Andrew J.
Bankart, John
Brightling, Christopher E.
O'Callaghan, Christopher L.
First Published: 5-Aug-2010
Publisher: Elsevier
Citation: Journal of Allergy and Clinical Immunology, 2010, 126 (4), pp. 722-729
Abstract: Background: Epithelial dysfunction has been implicated in asthma pathophysiology, but no studies have directly assessed ciliary function in asthma. Objective: To study the ciliary function and epithelial ultrastructure of patients with asthma and healthy controls. Methods: We studied ciliary beat frequency and beat pattern by using digital high-speed video imaging and ultrastructure by transmission electron microscopy of bronchial epithelial strips from 7 subjects with mild, 7 with moderate, and 19 with severe asthma and 9 healthy controls. Results: The median (interquartile range) ciliary beat frequency was decreased in moderate (6.5 [4.4-8.5] Hz) and severe asthma (6.7 [6.1-7.6] Hz) compared with controls (10.5 [9.7-11.8] Hz; P < .01). Dyskinesia and immotility indices were higher in severe asthma (65% [43%-75%]; 6.3% [1%-9.5%], respectively) compared with controls (4% [0%-6.7%; 0%, respectively; P < .01). These abnormalities were related to disease severity (ciliary beat frequency, rs = –0.68; dyskinesia index, rs = 0.86; immotility index, rs = 0.65; P < .0001). The ultrastructure of the epithelium was abnormal in severe asthma with a reduction in ciliated cells, an increase in dead cells, and ciliary disorientation compared with all other groups (P < .05). Compared with patients with mild asthma and healthy controls, patients with severe asthma showed increased ciliary depletion, microtubular defects, mitochondrial damage, and cytoplasmic blebbing (P < .01). All of these changes were related to disease severity. Conclusion: Ciliary dysfunction and ultrastructural abnormalities are closely related to asthma severity. Ciliary dysfunction is a feature of moderate to severe asthma, and profound ultrastructural abnormalities are restricted to severe disease. Whether these changes contribute to the development of severe asthma phenotype remains to be determined.
ISSN: 0091-6749
Links: http://www.sciencedirect.com/science/journal/00916749
http://dx.doi.org/10.1016/j.jaci.2010.05.046
http://hdl.handle.net/2381/8863
Type: Article
Description: Full text of this item is not currently available on the LRA. The final published version is available at http://www.sciencedirect.com/science/journal/00916749, Doi: 10.1016/j.jaci.2010.05.046.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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