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Title: Temperature-Dependent Modulation of Chromosome Segregation in msh4 Mutants of Budding Yeast
Authors: Chan, Andrew Chi-Ho
Borts, Rhona H.
Hoffmann, Eva R.
First Published: 9-Oct-2009
Publisher: Public Library of Science
Citation: PLoS ONE, 2009, 4(10), pp. e7284.
Abstract: Background: In many organisms, homologous chromosomes rely upon recombination-mediated linkages, termed crossovers, to promote their accurate segregation at meiosis I. In budding yeast, the evolutionarily conserved mismatchrepair paralogues, Msh4 and Msh5, promote crossover formation in conjunction with several other proteins, collectively termed the Synapsis Initiation Complex (SIC) proteins or ‘ZMM’s (Zip1-Zip2-Zip3-Zip4-Spo16, Msh4-Msh5, Mer3). zmm mutants show decreased levels of crossovers and increased chromosome missegregation, which is thought to cause decreased spore viability. Principal Findings: In contrast to other ZMM mutants, msh4 and msh5 mutants show improved spore viability and chromosome segregation in response to elevated temperature (23°C versus 33°C). Crossover frequencies in the population of viable spores in msh4 and msh5 mutants are similar at both temperatures, suggesting that temperature-mediated chromosome segregation does not occur by increasing crossover frequencies. Furthermore, meiotic progression defects at elevated temperature do not select for a subpopulation of cells with improved segregation. Instead, another ZMM protein, Zip1, is important for the temperature-dependent improvement in spore viability. Conclusions: Our data demonstrate interactions between genetic (zmm status) and environmental factors in determining chromosome segregation.
DOI Link: 10.1371/journal.pone.0007284
ISSN: 1932-6203 (Online)
Type: Article
Rights: This is the original published article published in PLoS ONE, 2009, 4(10), pp. e7284. The article is also available on open access from the publisher's webiste: doi:10.1371/journal.pone.0007284
Appears in Collections:Published Articles, Dept. of Genetics

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