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Title: Epistatic interactions of spontaneous mutations in haploid strains of the yeast Saccharomyces cerevisiae
Authors: Wloch, Dominika M
Borts, Rhona H.
Korona, Ryszard
First Published: Mar-2001
Publisher: Wiley-Blackwell
Citation: Journal of Evolutionary Biology, 2001, 14(2), pp.310-316.
Abstract: Several important biological phenomena, including genetic recombination and sexual reproduction, could have evolved to counteract genome contamination by deleterious mutations. This postulate would be especially relevant if it were shown that deleterious mutations interact in such a way that their individual negative effects are reinforced by each other. The hypothesis of synergism can be tested experimentally by crossing organisms bearing deleterious mutations and comparing the ®tness of the parents and their progeny. The present study used laboratory strains of the budding yeast burdened with mutations resulting from absence of a major DNA mismatch repair function. Only in one, or possibly two, crosses out of eight did ®tness of the progeny deviate from that of their parents in a direction indicating synergism. Furthermore, the distributions of progeny ®tness were not skewed as would be expected if strong interactions were present. The choice of experimental material ensured that genetic recombination was extensive, all four meiotic products were available for ®tness assays, and that the mutations were probably numerous. Despite this generally favourable experimental setting, synergism did not appear to be a dominating force shaping ®tness of yeast containing randomly generated mutations.
ISSN: 1010-061X
Type: Article
Description: The full text of this article is not available on the LRA. The published version can be found at DOI: 10.1046/j.1420-9101.2001.00267.x
Appears in Collections:Published Articles, Dept. of Genetics

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