Please use this identifier to cite or link to this item:
Title: Separable roles for exonuclease I in meiotic DNA double-strand break repair
Authors: Keelagher, Rebecca E.
Cotton, Victoria E.
Goldman, Alastair S. H.
Borts, Rhona H.
First Published: 1-Nov-2010
Publisher: Elsevier
Citation: DNA repair, 2011, 10(2), pp. 126-137.
Abstract: Exo1 is a member of the Rad2 protein family and possesses both 5' –3' exonuclease and 5 flap endonuclease activities. In addition to performing a variety of functions during mitotic growth, Exo1 is also important for the production of crossovers during meiosis. However, its precise molecular role has remained ambiguous and several models have been proposed to account for the crossover deficit observed in its absence. Here, we present physical evidence that the nuclease activity of Exo1 is essential for normal 5' –3' resection at the Spo11-dependent HIS4 hotspot in otherwise wild-type cells. This same activity was also required for normal levels of gene conversion at the locus. However, gene conversions were frequently observed at a distance beyond that at which resection was readily detectable arguing that it is not the extent of the initial DNA end resection that limits heteroduplex formation. In addition to these nuclease-dependent functions, we found that an exo1-D173A mutant defective in nuclease activity is able to maintain crossing-over at wild-type levels in a number of genetic intervals, suggesting that Exo1 also plays a nuclease-independent role in crossover promotion.
DOI Link: 10.1016/j.dnarep.2010.09.024
ISSN: 1568-7864
Type: Article
Rights: This is the authors' final draft of the paper published as DNA repair, 2011, 10(2), pp. 126-137. The original published version can be found on publisher's website at , DOI:10.1016/j.dnarep.2010.09.024
Appears in Collections:Published Articles, Dept. of Genetics

Files in This Item:
File Description SizeFormat 
DNAR Borts final draft.pdf1.45 MBAdobe PDFView/Open

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.