Please use this identifier to cite or link to this item:
|Title:||Inhibition of the Contractile Responses of Isolated Human and Rat Bladders by Clenbuterol.|
Elliott, Ruth A.
Terry, Tim R.
Sandhu, Davinder P.
Norman, Robert I.
|Publisher:||Elsevier on behalf of the American Urological Association.|
|Citation:||Journal of Urology, 2001, 166 (5), pp. 1969-1973.|
|Abstract:||Purpose: We investigated the inhibition of the contractile responses of human continent and unstable detrusor muscle by the β2 agonist clenbuterol as well as the inhibition of electrical field stimulation evoked contractile responses of isolated rat bladder muscle strips by orally administered clenbuterol. Materials and Methods: The contractile responses of human continent and unstable detrusor muscle strips to electrical field stimulation (0.05 milliseconds, 0.5 to 80 Hz.) were measured before and after adding 10−9 to 10−4 M. clenbuterol in vitro. In addition, 6 rats per group were dosed orally with 2 μg. kg.−1 clenbuterol daily acutely (1 dose) or chronically (1 dose daily for 8 days), or with distilled water to serve as controls. The contractile response to electrical field stimulation of strips of isolated detrusor muscle was then measured. Serum clenbuterol levels were analyzed in duplicate by enzyme-linked immunosorbent assay and high performance liquid chromatography. Results: In vitro clenbuterol significantly inhibited the electrical field stimulation evoked contractile responses of detrusor muscle strips from unstable but not continent human bladders. A significant inhibitory effect of clenbuterol on the electrical field stimulation evoked contractile response of rat detrusor muscle was observed after chronic but not acute oral dosing (p <0.01). Serum clenbuterol levels measured by enzyme-linked immunosorbent assay and high performance liquid chromatography were not significantly different. Conclusions: Clenbuterol or related β2-adrenoceptor agonists may represent a useful therapeutic strategy for detrusor muscle overactivity.|
|Description:||This paper was published as Journal of Urology, 2001, 166 (5), pp. 1969-1973. It is available from http://www.jurology.com/article/S0022-5347(05)65729-1/abstract.|
Metadata only entry
|Appears in Collections:||Published Articles, Dept. of Cancer Studies and Molecular Medicine|
Files in This Item:
There are no files associated with this item.
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.