Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/9919
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dc.contributor.advisorPritchard, Catrin-
dc.contributor.advisorBeck, Felix-
dc.contributor.authorStringer, Emma Jane-
dc.date.accessioned2011-11-25T10:54:49Z-
dc.date.available2011-12-16T02:45:02Z-
dc.date.issued2008-12-16-
dc.identifier.urihttp://hdl.handle.net/2381/9919-
dc.description.abstractIt has been suggested that the intestinal endoderm is responsive to signals received from underlying mesoderm, indicating cross-talk between the layers. Cdx2 mutant mice develop heterotopias of stomach-type epithelium within the paracaecal region of the intestine. They are therefore an ideal in-vivo model in which to study whether the loss of endodermal gene expression required for intestinal development leads to stomach-specific mesodermal gene expression. In this study, Sox2, a stomach endoderm-specific marker, was used to detect gastric heterotopias in Cdx2 mutant embryonic intestine using whole-mount in-situ hybridisation. The nature of the underlying mesoderm was investigated using a second marker, Barx1, which is known to be specifically expressed in the stomach mesoderm. RT-PCR was used to detect low levels of Barx1 expression in Cdx2+/- caecum samples. Further investigation using in-situ hybridisation techniques indicated regions of Barx1 expression in a similar distribution to the regions detected using the Sox2 probe. This finding confirms that the mesoderm underlying the Cdx2 mutant gastric heterotopias expresses a stomach-specific gene and therefore that the mesoderm is responsive to endodermal signals. It has been suggested that Cdx2+/- mice do not develop gastric-type intestinal heterotopias postnatally. If proven, this would indicate that intestinal stem cell potential becomes limited at some point during development and prevents the epithelium responding to a postnatal loss of Cdx2 protein. A conditional Cdx2 mouse model is required in order to investigate this hypothesis. The creation of this mouse model formed the second part of this project. Following creation of a targeting vector, transfection into ES cells and injection into blastocysts, chimeric mice were obtained. These mice were successfully bred for germline transmission.en
dc.language.isoenen
dc.rightsCopyright © the author, 2008.en
dc.titleAn investigation of endoderm to mesoderm signalling in gut developmenten
dc.typeThesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhDen
dc.date.award2008-12-16en_GB
dc.publisher.institutionUniversity of Leicesteren_GB
Appears in Collections:Theses, Dept. of Biochemistry
Leicester Theses

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